Select keep source images to keep original images open. Determine which image will be which channel (red, green, blue, cyan etc.) and merge using the dropdown menu.
#Merge images imagej 64 Bit#
If you are unsure if you have a 64 bit or a 32 bit OS, type “See if you have a 32-bit or 64-bit OS” into your computer’s search bar or go to Computer Settings > About.
#Merge images imagej download#
Determine the appropriate download for your operating system. To download Fiji, go to and scroll until you see the download section.
Select one of the images and choose 'merge channels' from the channel palette menu. If you have only two images to merge, open a third anyway just as a placeholder. (Go to Image>Mode and choose grayscale if this is not already the case). For further instruction, please refer to the NIH’s ImageJ user guide and other Fiji documentation. They should be saved as TIFF and the mode should be set to grayscale. This brief guide will outline several basic processing and analysis methods.